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High throughput protein expression and purification : methods and protocols
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| 目次 | High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production Jim Koehn and Ian Hunt Designing experiments for high-throughput protein expression Stephen P. Chambers and Susanne E. Swalley Gateway cloning for protein expression Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath Flexi vector cloning Paul G. Blommel ... [et al.] The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning Nick S. Berrow, David Alderton, and Raymond J. Owens The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis Heath E. Klock and Scott A. Lesley A family of LIC vectors for high-throughput cloning and purification of proteins William H. Eschenfeldt ... [et al.] "System 48" high-throughput cloning and protein expression analysis James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery E. coli and insect cell expression, automated purification and quantitative analysis StephenP. Chambers ... [et al.]. Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh PHB-intein-mediated protein purification strategy Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood High-throughput biotinylation of proteins Brian K. Kay, Sang Thai, and Veronica V. Volgina High-throughput insect cell protein expression applications Mirjam Buchs ... [et al.] High-throughput protein expression using cell-free system Kalavathy Sitaraman and Deb K. Chatterjee The production of glycoproteins by transient expression in mammalian cells Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens High-throughput expression and detergent screening of integral membrane proteins Said Eshaghi Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform Jenny A. Cappuccio ... [et al.] Expression and purification of soluble His₆-tagged TEV protease Joseph E. Tropea, Scott Cherry, and David S. Waugh High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation Priscilla A. Moore and Vladimir Kery.続きを見る |
| 本文を見る | Springer Nature Complete Protocols: 2009 |
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| 登録日 | 2023.09.29 |
| 更新日 | 2024.01.30 |