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Membrane trafficking
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| 概要 | This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and syne...rgism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells. This volume contains updated chapters and the inclusion of a large number of new chapters, and is divided into three parts. Part I includes biochemical and molecular genetics approaches and methods used in analysing membrane traffic, in both yeast and mammalian cell models. Part II focuses on imaging and microscopy approaches and techniques, while Part III highlights the Omics-type approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and resourceful, Membrane Trafficking, Second Edition, is a valuable resource for both experienced researchers, graduate students, and junior scientists in the field of membrane trafficking.続きを見る |
| 目次 | Intracellular parcel service : current issues in intracellular membrane trafficking Johannes M. Herrmann and Anne Spang In vitro analysis of the mitochondrial preprotein import machinery using recombinant precursor polypeptides Dorothea Becker and Wolfgang Voos Import of proteins into isolated yeast mitochondria Valentina Peleh, Ajay Ramesh, and Johannes M. Herrmann Evaluation of unconventional protein secretion in Saccharomyces cerevisiae Natsuko Miura and Mitsuyoshi Ueda Fractionation of plasmodium-infected human red blood cells to study protein trafficking Simone Külzer, Verena Bittl, and Jude M. Przyborski Investigating signaling processes in membrane trafficking Laura J. Sharpe and Andrew J. Brown Recruitment of coat proteins to liposomes and peptidoliposomes Sonja Huser, Gregor Suri, Pascal Crottet, and Martin Spiess A [beta]-lactamase based assay to measure surface expression of membrane proteins Pieter Beerepoot, Vincent M. Lam, and Ali Salahpour Cell-free reconstitution of multivesicular body (MVB) cargo sorting Monica Gireud, Natalie Sirisaengtaksin, Susan Tsunoda, and Andrew J. Bean Analysis of biogenesis of lipid droplets by examining Rab40c associating with lipid droplets Ran Tan, Xiaohui Xu, Wanjin Hong, and Tuanlao Wang Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins Heon yung Gee, Joo Young Kim, and Min Goo Lee Assessing mammalian autophagy Sharon A. Tooze, Hannah C. Dooley, Harold B.J. Jefferies, Justin Joachim, Delphine Judith, Christiopher A. Lamb, Minoo Razi, and Martina Wirth Expression of functional myc-tagged conserved oligomeric golgi (COG) subcomplexes in mammalian cells Rose A. Willett, Tetyana A Kudlyk, and Vladimir V. Lupashin Molecular and cellular characterization of GCC185 : a tethering protein of the trans-golgi network Pak-yan Patricia Cheung and Suzanne R. Pfeffer Visualizing toll-like receptor-dependent phagosomal dynamics in murine dendritic cells using live cell microscopy Adriana R. Mantegazza and Michael S. Marks Understanding of complex protein interactions with respect to anchorage independence Jill M. Tham, Sofie C. Van Huffel, and Wanjin Hong Application of flow cytometry to analyze intracellular location and trafficking of cargo in cell populations Wei Hong Toh, Fiona J. Houghton, Pei Zhi Cheryl Chia, Yasmin M. Ramdzan, Danny M. Hatters, and Paul A. Gleeson Approaches to analyze the role of Rab GTPases in endocytic trafficking of epidermal growth factor receptor (EGFR) Christelle En Lin Chua, Angeline Yim Kiat Lim, and Bor Luen Tang Does super-resolution fluorescence microscopy obsolete previous microscopic apporaches to protein co-localization? Laura MacDonald, Giulia Baldini, and Brian Storrie Bimolecular fluorescence complementation (BiFC) technique in yeast Saccharomyces cerevisiae and mammalian cells Marion Weber-Boyvat, Shiqian Li, Kari-Pekka Skarp, Vesa M. Olkkonen, Daoguang Yan, and Jussi Jäntti Microscopic and spectroscopic techniques to investigate lipid droplet formation and turnover in yeast Heimo Wolinski and Sepp D. Kohlwein Image-based identification of nuclear export inhibitors from natural products Bastien Cautain, Nuria de Pedro, Fernando Reyes, and Wolfgang Link Correlative video-light-electron microscopy of mobile organelles Galina V. Beznoussenko and Alexander A. Mironov Live cell imaging of endosomal trafficking in fungi Sebastian Baumann, Norio Takeshita, N. Grün, Reinhard Fischer, and Michael Feldbrügge Quantitative analysis of transferrin cycliing by automated fluorescence microscopy David T. Hirschmann, Christoph A. Kasper, and Martin Spiess Identification of factors regulating MET receptor endocytosis by high-throughput siRNA screening Ivana Gaziova, Robert A. Davey, and Lisa A. Elferink Large-scale analysis of membrane transport in yeast using invertase reporters Lauren Dalton, Michael Davey, and Elizabeth Conibear RNAi screens for genes involved in Golgi glycosylation Germaine Y. Goh and Frederic A. Bard Proteomic analyses of a bi-lobed structure in Trypanosoma brucei Ladan Gheiratmand and Cynthia Y. He Application of the proximity-dependent assay and fluorescence imaging approaches to study viral entry pathways Alex Lipovsky, Wei Zhang, Akiko Iwasaki, and Daniel DiMaio.続きを見る |
| 本文を見る | Springer Nature Complete Protocols: 2015 |
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| 登録日 | 2023.09.29 |
| 更新日 | 2024.01.30 |