Effect of inhibitors on the inorganic nitrogen reductases from fowl liver were studied. The rate of inhibition of the reductases by several reagents was as follows. Hydroxylamine : Thiourea, EDTA (2.5 × 10-3M), 8-Hydroxyquinoline (2.5 × 10-4M) ......... 0 % Uric acid (2.5 × 10-4M)......... 30 % KCN (2.5 × 10-3M)......... 70 % PCMB (1.0 × 10-4M).........100 % Hyponitrite : PCMB (1.0 × 10^-4M)......... 0% EDTA (2.5 × 10^-3M), 8-Hydroxyquinoline (2.5 × 10^-4M)......... 50 % KCN (2.5 × 10^-3M)......... 60 % Thiourea (2.5 × 10^-3M), Uric acid (2.5 × 10^-4M).........100 % Nitrite : KCN (2.5 × 10^-3M)......... 0 % 8-Hydroxyquinoline (2.5 × 10^-4M)......... 40 % Thiourea, EDTA (2.5 × 10^-3M), PCMB (1.0 × 10^-4M).........100 % Nitrate : EDTA, KCN (2.5 x 10^-3M) ......... 60- 70 % Thiourea (2.5 x 10^-3M), Uric acid, 8-Hydroxyquinoline (2.5 x 10^-4M), PCMB(1.0 x 10-4M) ......... 85～100 % 2) Hyponitrite was decomposed completely by the addition of glacial acetic acid without producing any nitrite as well as by heating at 100℃. On the other hand, there was no decomposition of hyponitrite by uranium acetate which was used for the deproteinization. 3) Hyponitrite was determined as the reducing product of nitrite. Although hyponitrite was not detected in the normal reduction of nitrite, it can be estimated when KCN, a specific inhibitor for its reduction, was added to the reaction mixture. 4 ) The improved method for the synthesis of a-ketoglutarate oxime was described.
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