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＜departmental bulletin paper＞
Application of T-RFLP Analysis for Bacterial Community Structure of Colonies Grown on Agar Plates

Creator
Creator Name
Matsuka, Akira
松家, 輝
マツカ, アキラ
Affiliation
Affiliation Name
Laboratory of Soil Microbiology,Division of Soil Science and Plant Production,Department of Plant Resoruces,Graduate School of Bioresource and Bioenvironmental Sciences,Kyushu University
Creator Name
Sakai, Masao
境, 雅夫
サカイ, マサオ
Affiliation
Affiliation Name
Laboratory of Soil Microbiology,Division of Soil Science and Plant Production,Department of Plant Resources,Faculty of Agriculture,Kyushu University
Author PID
K000392
Creator Name
Kanawaza, Shinjiro
金澤, 晋二郎
カナザワ, シンジロウ
Affiliation
Affiliation Name
Laboratory of Soil Microbiology,Division of Soil Science and Plant Production,Department of Plant Resources,Faculty of Agriculture,Kyushu University
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2003-10-01
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
48
Issue
1/2
First Page
107
Last Page
112
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/4532
Related DOI
Journal of the Faculty of Agriculture, Kyushu University || 48(1/2) || p107-112
九州大学大学院農学研究院紀要 || 48(1/2) || p107-112
http://www.agr.kyushu-u.ac.jp/index.html
Related URI
Journal of the Faculty of Agriculture, Kyushu University || 48(1/2) || p107-112
九州大学大学院農学研究院紀要 || 48(1/2) || p107-112
http://www.agr.kyushu-u.ac.jp/index.html
Relation
Journal of the Faculty of Agriculture, Kyushu University || 48(1/2) || p107-112
九州大学大学院農学研究院紀要 || 48(1/2) || p107-112
http://www.agr.kyushu-u.ac.jp/index.html

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
4532
Peer-Reviewed
Unrefereed
ISSN
0023-6152
NCID
AA00697606
Notes
As a prompt and efficient method for analysis of community structure of bacterial colonies grown on agar plants, we investigated the application of terminal restriction fragment length polymorphism (T-RFLP) analysis. To deduce the community structure of colonies grown on agar olants, analysis of a number of colonies is necessary. In this analytical method, many colonies were collectively subjected to T-RFLP analysis to estimate the community structure of colonies. To analyze dominant bacterial soecies in test soils, 100 colonies were sufficient. Using this analytical method, we investigated the influence of planting method for colony count (pour-plate method vs. spread-plate method) on the constitution of bacterial species grown on the medium. Difference in plamting method did not markedly change the bacterial species grown, but it did affect the constitution ratio of some bacterial species.
Created Date 2009.04.22
Modified Date 2022.11.11

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