| 作成者 |
|
|
|
| 本文言語 |
|
| 出版者 |
|
| 発行日 |
|
| 収録物名 |
|
| 巻 |
|
| 開始ページ |
|
| 終了ページ |
|
| 出版タイプ |
|
| アクセス権 |
|
| JaLC DOI |
|
| 関連DOI |
|
|
|
| 関連URI |
|
|
|
| 関連情報 |
|
|
|
| 概要 |
To perform the genotyping method of Staphylococcus aureus based on random amplified polymorphic DNA analysis (RAPD), we optimized the several polymerase chain reaction (PCR) conditions such as Mg2+ co...ncentration, annealing temperature and DNA polymerase. In order to assess the discriminatory power of this typing method, 18 epidemiologically unrelated S. aureus strains were examined and the results were compared with the coagulase gone typing method. Among the five primers, RAPD PCR using primer 7 yielding 15 different types was the most discriminative primer. All the strains except two were distinguished each others by the combination of RAPD profiles acquired using the three primers.These data suggest that the discriminatory power of the RAPD genotyping method is great enough to be used as the sole method to type S. aureus and this method is useful as an epidemiological tool.続きを見る
|
Mendeley出力
KJ00000075240-00001