The temperature at which the highest photosynthetic rate of Vitis species was obtained was at a range of 20-30℃, though the optimum temperature varied depending on the species. The photosynthetic rate obviously declined at lower or higher temperature than that. Transpiration rates of the species generally increased as temperature increased, and the rate was the highest between 30-35℃. Stomatal conductivity, CO_2 concentration inside cell spaces and water-use efficiency reached the maximum at 15-20℃. Thereafter, they decreased as temperature increased. The photosynthetically optimum temperature zone of the wild species grown in cool regions or higher latitudes was in low temperature one. In contrast, that of the species grown in warm regions or lower latitude was in high temperature zone. Therefore, the photosynthetic response to temperature might be closely related to the temperature in the sourse regions. Leaf thickness, dry weight per unit leaf area, percentage of dry matter, chlorophyll content, stomatal length and stomatal density varied depending on the species. We found no close relationship among the photosynthetic rate, transpiration rate, stomatal conductivity, CO_2 concentration in cell space, water-use efficiency and shape of the leaf.