Feeding trials were conducted in order to compare the availability of carbohydrate and lipid as dietary energy sources for puffer fish. The fish were fed diets containing different amounts of carbohydrate (30-7 %) and lipid (6-22%) at a fixed protein level of 50%. The growth performance was examined in terms of weight gain, feed efficiency, protein efficiency ratio and body composition for 8-week feeding trials at 22.5-28.0℃. The average weight gain as well as feed efficiency, protein efficiency ratio and protein retention rate increased in fish given the diets with lower carbohydrate and higher lipid levels. Proximate analysis of body composition showed that dorsal muscle was not affected by dietary lipid level. However, moisture and crude protein in the liver were negatively correlated and crude lipid was positively correlated to dietary lipid level. It could be concluded from this study that lipid was more available as dietary energy source than carbohydrate.