The major anthocyanin pigments from Bailey Alicante A grapes have been separated using column chromatography on insoluble polyvinylpyrolidone (PVP) and polyamide C-200 (PA) to obtain the pure pigments in quantities large enough for chemical research. 1. The adsorption of pigment to adsorbents increased with increasing pH (pH≦7.0). The recovery of pigment from pigment-PVP and pigment-PA adsorbates was 50 % and 80 %, respectively. 2. A stepwise elution of the prepared pigments on PA-column (0. 1 % HCl-H_2O and 0. 1 % HCl-20 % EtOH) and PVP-column (0. 1 % HCl-30 % EtOH and 0. 1 % HCl-80 EtOH) gave four pigment fractions, respectively. 3. An elution of the anthocyanin pigments in grape juice on PA-column resulted in poorer resolution than that on PVP-column. Using a gradient elution on PA-column, the anthocyanins of Bailey Alicnate A grape juice were fractionated four groups according to their degree of glycosylation and acylation with p-coumaric acid. 4. From these results, the separation of individual pigments using column chromatography on PA and PVP was discussed.