In the glucose tolerance test (GTT) and insulin glucose tolerance test (IGTT) with fish, the change of blood sugar content is determined by a repeated bleeding method or a one-time bleeding method. It is desirable that the change of blood sugar is found through samples of blood taken repeatedly at regular intervals from a given fish. It is to be expected that the blood sugar will increase in proportion to the rapid shock caused by handling and repeated bleeding. The present study, therefore, w as carried out to determine the influence of repeated bleeding and anesthetization on the change of blood sugar content of the red sea bream (Chrysophrys major), to determine the difference of influences among anesthetics, and to compare the change of blood sugar content in the GTT and IGTT which was obtained by a repeated bleeding method and that obtained by a one-time bleeding method. The results obtained can be summarized as follows: 1) The blood sugar content of the red sea b ream determined by repeated bleeding without anesthetization increased remarkably each time a bleeding was taken, and there was a large difference between the change of blood suger content in individual fish. 2) The increase of blood sugar obtained by repeated bleeding on a given fish anesthetized with urethane (5,000 ppm), or quinaldine (20 ppm), or MS-222 (100 ppm) was lower than that obtained by repeated bleeding without anesthetization. The increase of blood sugar obtained by repeated bleeding with MS-222 anesthetization was the least. 3) The average blood sugar content obtained from three different fish at each t ime by the one-time bleeding method with urethane anesthetization was always in the range from 50-75 mg/dl. 4) The changes of blood sugar content in the GTT and IGTT obtained from a given fish by repeated bleeding with MS-222 anesthetization showed the same curve as those obtained from numerous fish by a one-time bleeding method with urethane anesthetization, but the quantities of increase and decrease of blood sugar content obtained by both methods were different.
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