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＜博士論文＞
ショウジョウバエ概日時計に関与する膜タンパク質の行動分子遺伝学的解析
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| 概要 | ATP-binding cassette transporters (ABC transporters) are a kind of membrane proteins, which bind ATP and use the energy to drive the transport of various molecules across all cell membranes. The funct...ions are widespread and well-conserved among organisms. In Drosophila melanogaster, there are 56 genes encoding ABC transporters. In this study, we examined whether there is a clock-related ABC transporter by performing a genome-wide screen with tissue-specific RNA interference (RNAi). We obtained five candidates with tim(UAS)-gal4 in which all clock-related cells virtually express GAL4. Since their phenotypes were principally reproducible even with pdf-gal4 in which only a subset of pacemaker neurons express GAL4, those transporters were presumed to function in pacemaker neurons. Those five candidates can be categorized into two groups according to the phenotype of knockdown flies. In one group, CG9281 and CG15410 (E23), knockdown flies altered the circadian period. In the other group, CG5944, CG6052 and CG18633, a part of the knockdown flies became arrhythmic while the remaining part kept an intact rhythmicity. Our results imply that at least these five ABC transporters have a significant function in the Drosophila circadian system. Especially, one of the genes, Early gene at 23 (E23) is induced by the molting hormone ecdysone. Pacemaker neurons in fly head express E23, and its knockdown flies lengthened circadian period with an increased expression of the clock gene vrille. E23 and vrille responded to both ecdysone and clock signals, whereas E23 protein specifically suppressed the ecdysone response and is necessary for rhythmicity. Thus, E23 forms its own feedback loop in the ecdysone response to control circadian oscillation through ecdysone-mediated vrille expression. The ecdysone signaling pathway involving E23 is essential not only in developmental stage but also for the circadian behavior in adult fly.続きを見る |
| 目次 | Acknowledgments Abstract Introduction Materials and Methods Results Discussion Tables Figures References |
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| 登録日 | 2013.07.12 |
| 更新日 | 2023.11.21 |