The present paper deals with the growth-promoting effect of "solubilized fish" and its active ingredient upon the growth of a plant, Lemna paucicostata Hegelm. The solubilized fish used has been prepared by the following procedure. A ground fish body after being acidified at pH 4.0～4.5, was subjected to autolysis at 50℃. The liquefied part was separated from the residue by filtration and then neutralized. The concentration of the plant growth factor in the solubilized fish has been attempted and realized by the following procedure. The solubilized fish solution after its pH being adjusted at 4.5, was shaken with the active carbon for 30 min. and filtered. The active factor was eluted with a dilute solution of sodium hydroxide. It has been shown that the solubilized fish is effective to the growth of the plant ( Table 1 and Fig. 1). The growth factor is not an inorganic matter and seems not to be a secondary product formed from the solubilized fish by bacteria during the culture ( Table 2 ). The data presented here show that the fraction adsorbed by the active carbon is very effective to the growth of the plant, whereas the non-adsorbed fraction remains ineffective ( Table 4 and 6; Fig. 2, 3 and 4 ).
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