| 概要 |
We used immunohistochemical techniques to analyze the localization and distribution of the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and the neuropeptides methionine-enkephalin (M-En...k), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calretinin (Cal), calcitonin gene-related peptide (CGRP), substance P (SP), and galanin (Gal) in the stellate ganglia of two species of domestic animal (cattle and horses). NPY, VIP and Gal immunoreactive neurons (both cell body and nerve fiber) were observed in the stellate ganglia of both animals. M-Enk and CGRP immunoreactive nerve fibers were detected in the stellate ganglia of both animals, but positive cell bodies were found only in the stellate ganglia of the horse. In contrast, Cal- and SP-positive cell bodies existed in the stellate ganglia of cattle but not in the horse. SP-immunoreactive cells were observed only in young cattle. In both cattle and horses, almost all ganglion cell bodies exhibited TH immunoreactivity, but no positive nerve fibers were found.
ウマとウシの星状神経節内の神経ペプチドを免疫組織化学的に検索した.食肉衛生検査所および学内で採取したウシ,ウマの星状神経節について常法に従い,凍結切片を作成し,市販されている抗体を用いて神経節内のペプチドの検索を免疫組織化学的に行った.7種類の神経ペプチドの抗体を用いて観察した結果,今回検索したペプチドはすべて神経節内の神経線維に対して陽性反応を示した.M-EnkとCGRPについては,ウマでは細胞体及び線維とも反応陽性であったが,ウシでは神経線維は陽性反応を示し,細胞体は反応陰性であった.SPとCalでは逆にウシの星状神経節のみに反応陽性細胞が見られた.ウシでのSP反応陽性細胞は若齢のもののみ見られた.NPY,Gal,VIPはこれまでの報告と一致して,神経線維,神経細胞両者で反応陽性であった.THについては神経節細胞体のほとんどが陽性反応を示した.続きを見る
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Mendeley出力
fam94_12_p338